PENGARUH PEMBERIAN KARBON TETRAKLORIDA, NIASIN, KAFEIN, DAN MADU TRIGONA TERHADAP FUNGSI HATI TIKUS

• Dian Aulia Istiqomah

  Universitas Wijaya Kusuma Surabaya

  Author

Karbon tetraklorida adalah zat yang biasa digunakan untuk menginduksi peroksidasi lipid dan keracunan. Akhir-akhir ini, terdapat laporan mengenai kasus hepatotoksisitas yang disebabkan oleh minuman berenergi dan kandungan niasin didalamnya diduga berperan sebagai penyebab kerusakan hati. Kandungan kafein yang ada didalam minuman berenergi sebenarnya memiliki efek hepatoprotektif. Namun, efek hepatoprotektif kafein kurang kuat dalam menangkal efek hepatotoksik dari niasin. Kerusakan hati dideteksi melalui peningkatan enzim transaminase. Madu trigona mengandung flavonoid yang tinggi dan memiliki peran protektif terhadap inflamasi dan stres oksidatif. Selain enzim transaminasi, salah satu indikator gangguan fungsi ginjal yaitu dengan adanya peningkatan kreatinin serum. Karbon tetraklorida dosis 0,1 dan 1,0 ml/kg bobot badan mengakibatkan peningkatan kreatinin, sebaliknya pada dosis 10 ml/kg bobot badan kadar kreatinin sudah sangat turun (p<0,05). Sehingga, Karbon tetraklorida menimbulkan kerusakan sebanding dengan dosis yang diinduksikan. Serta tidak ada korelasi yang signifikan pada kadar enzim AST dan ALT (p>0,05). Sebaliknya, ada  korelasi yang signifikan pada kadar enzim GGT (p < 0,05). Studi ini menunjukkan bahwa niasin dan kafein dosis 0,491 mg/200grBB/hari dan 1,2275 mg/200grBB/hari dalam model minuman berenergi yang setara dengan mengkonsumsi tiga kemasan per hari dapat meningkatkan kadar enzim GGT. Selain itu, medu trigona juga memiliki efek hepatoprotektor yang berfungsi untuk menurunkan kadar ALT dan AST.

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